Monkeypox Virus Detection Kit (Fluorescence PCR) Instructions (Part 2)

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[Kit Components] 

REF: AMPV01

Components

Quantity

48 tests/kit

96 tests/kit

MPV   B-S

500μL/tube,   1 tube

1000μL/tube,   1 tube

MPV   P-P

250μL/tube,   1 tube

500μL/tube,   1 tube

MPV   N Ctrl

1000   μL/tube, 1 tube

1000μL/tube,   1 tube

MPV   P Ctrl

1000   μL/tube, 1 tube

1000μL/tube,   1 tube

REF: AMPV01D

Components

Quantity

48 tests/kit

96 tests/kit

MPV   Mix

Lyophillization,   48 tubes

Lyophillization,   96 tubes

MPV   N Ctrl

400   μL/tube, 1 tube

400   μL/tube, 1 tube

MPV   P Ctrl

Lyophillization,   1 tube

Lyophillization,   1 tube

[Storage] 

The kit with REF: AMPV01 is stable when stored at -25°C to -15°C for 12 months. Please transport at -25℃ to -15℃. 

The kit with REF: AMPV01D is stable when stored at below 35°C for 18 months. 

The kits should remain sealed and away from light exposure. Do not use the kit beyond the expiration date.

[Applicable Instruments] 

Recommended qPCR instruments include: Anitoa Maverick qPCR systems series. Bio-Rad CFX96 Touch Real-Time PCR Detection System; Applied Biosystems™ 7500 real-time PCR system; Applied Biosystems™ QuantStudio 5 real-time PCR system; Applied Biosystems™ QuantStudio 3 real-time PCR system; Applied Biosystems™ QuantStudio 7 Flex/Pro real-time PCR system; Applied Biosystems™ ViiA7 real-time PCR system; Applied Biosystems™ StepOnePlus™ Real-Time PCR System.

[Materials Required but Not Provided] 

1. 48 or 96-well Polypropylene PCR Microplate. Or 0.2 mL Polypropylene PCR Tube Strips with Flat Cap 

2. Preservation solution 

3. Pipettes and Pipetting tips (10μL and 200μL tips with filters) 

4. Centrifuge 5. Desktop vortex mixer 

6. Disposable powder-free gloves and surgical gowns

[Specimen Requirements] 

1. Sample type Oropharyngeal swab, nasopharyngeal swab, serum, plasma and human pustular or vesicular rash specimens. 

2. Specimen collection Human pustular or vesicular rash specimens can be collected in tube containing viral transport media with a swab. Oropharyngeal swab and nasopharyngeal swab are collected using a polypropylene fiber swab or flocked swab with plastic shafts.

Plasma- Centrifuging the anticoagulant blood at 1000g for 15min, and transfer supernatant to a new tube. 

Serum - Mix collection tube by inverting 5-8 times immediately after blood collection, then let stand for 30 minutes, centrifuge at 1500g for 10min.

3. Specimen storage conditions 

The collected specimens should be sent for testing immediately. Specimens should be tested within 24 hours if stored at 2°C to 8°C. Specimens that cannot be tested within 24 hours should be stored at -70°C or below (in the absence of -70°C storage conditions, specimens can be stored at -25°C to -15°C for 10 days). Multiple freeze/thaw cycles should be avoided. Specimens should be transported in a sealed frozen pitcher with ice or in a sealed foam box with ice.


Professional Portable qPCR System Manufacturer - Anitoa

Anitoa is a professional R&D and manufacturing company of molecular diagnostic qPCR instruments and reagents, with independent intellectual property rights of core chip technology, optical technology, rapid heating, microfluidic control and supporting reagents and other patented technologies, so that the instruments developed by Anitoa are characterized by quick and easy small instruments, allowing the development of expensive and complex large PCR instruments into truly portable POCT products.