Introduction of Nucleic Acid Detection Kit for African Swine Fever Virus
Product Information
Item No. | Product Name | Specification |
MUBP01 | African swine fever virus nucleic acid detection kit | 50T/100T/200T |
MUBP02 | African swine fever virus wild strain and gene deletion strain identification test kit | 50T/100T/200T |
Detection Target
African swine fever virus nucleic acid detection kit: the P72 gene with high sensitivity and good specificity is preferred, in addition, the porcine ATCB gene is used as internal quality control.
African swine fever virus wild strain and gene deletion strain identification test kit: preferably P72, CD2v, MGF genes recommended in the Technical Guide for Prevention and Control of African Swine Fever Virus Variant on Farms, in addition to pig ATCB genes as internal quality control.
Product Features
This product adopts qPCR technology, which has the advantages of high sensitivity, good specificity, fast isothermal amplification reaction speed and low cost of equipment.
Extraction-free reagents for testing serum or swab samples enable direct amplification without nucleic acid extraction.
The performance of the components is stable, and there is no change in performance when left at 4 °C for 12 months.
High sensitivity, the kit has high specificity and can detect single copy.
Good specificity, the kit has high specificity and does not cross-react with other common pig pathogens.
Good reproducibility, the kit has good reproducibility with intra-batch precision coefficient of variation <5%.
The reagents come with internal standards to monitor the entire sample collection, sample addition and amplification process. It can reduce the chance of system errors.
Anti-contamination, UNG enzyme + dUTP measures were introduced in the reagent to effectively degrade the aerosol contamination of amplification products and avoid false positives.
Short detection time, 30 minutes to complete a test to improve detection efficiency.
Reagent repeatability, linearity and sensitivity (Item No. MUBP01)
Repeatability（8000copies/rxn）
Linearity（8000~8copies/rxn）
Sensitivity（8copies/rxn）
Real sample test results
PCR amplification was performed on 8 tubes of different pig serum samples with African swine fever virus nucleic acid detection kit (Item No. MUBP01) and African swine fever virus wild strain and gene deletion strain identification kit (Item No. MUBP02), respectively, and the results of the two reagents on pig serum samples were highly consistent.
Professional Portable qPCR System Manufacturer - Anitoa
Anitoa is a professional R&D and manufacturing company of molecular diagnostic qPCR instruments and reagents, with independent intellectual property rights of core chip technology, optical technology, rapid heating, microfluidic control and supporting reagents and other patented technologies, so that the instruments developed by Anitoa are characterized by quick and easy small instruments, allowing the development of expensive and complex large PCR instruments into truly portable POCT products.